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ktrC [2019-05-08 09:46:51]
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ktrC [2019-05-08 09:46:51]

low affinity potassium channel KtrC-KtrD, peripheric membrane component
Locus
BSU14510
Isoelectric point
5.63
Molecular weight
24.20 kDa
Protein length
221 aa Sequence Blast
Gene length
666 bp Sequence Blast
Function
potassium uptake
Product
low affinity potassium channel KtrC-KtrD, peripheric membrane component
Essential
no
Synonyms
ylxV,yzaC,ykqB
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Cellular processes → Transporters → Transporters/ other → Metal ion transporter
  • Cellular processes → Homeostasis → Metal ion homeostasis (K, Na, Ca, Mg) → Potassium uptake/ export
  • Information processing → Targets of second messengers → Targets of c-di-AMP
  • Lifestyles → Coping with stress → Coping with hyper-osmotic stress
  • Groups of genes → Membrane proteins

    • Gene

    Coordinates
    1,520,531 1,521,196

    The protein

    Protein family

  • KtrA potassium transport family (with KtrA, according to UniProt)

    • Paralogous protein(s)

  • KtrA

    • Kinetic information

  • the KtrC-KtrD channel has a low affinity for potassium,this is determined by KtrD PubMed

    • Domains

  • contains a RCK_N domain at the N-terminus (according to UniProt)
  • contains a c-di-AMP-binding RCK_C domain at the C-terminus (according to UniProt) PubMed

    • Effectors of protein activity

  • binds ADP and ATP PubMed
  • the protein binds c-di-AMP, KD = 30 nM PubMed

    • Structure

  • 4J7C (the KtrA-KtrB complex, 56% identity) PubMed
  • 6I8V (KtrC in complex with ATP) PubMed
  • 4XTT (the S. aureus KtrA RCK_C domain in complex with c-di-AMP, 57% identity) PubMed

    • Localization

  • peripheral membrane protein PubMed

    • Expression and Regulation

    Operons

    Genes
    ktrC
    Description
    PubMed

    Regulation

  • constitutively expressed PubMed
    • view in new tab

    Genes
    kinC-ykqA-ktrC
    Description
    PubMed

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

    • Regulatory mechanism

  • Spo0A: activation, PubMed, in Spo0A regulon

    • Regulation

  • constitutively expressed PubMed
    • view in new tab

    Biological materials

    Mutant

  • MGNA-A904 (ykqB::erm), available at the NBRP B. subtilis, Japan
  • GHB6 (ktrC::spec), PubMed (available in Erhard Bremer's and Jörg Stülke's) labs, available at BGSC as 1A955
  • GP2264 (ΔktrC::aphA3), available in Jörg Stülke's lab PubMed
  • GP2048 (ΔktrC::cat), available in Jörg Stülke's lab PubMed
  • GP2079 (ΔktrC::tet), available in Jörg Stülke's lab PubMed
  • GP2771 (ΔktrC::spec), available in Jörg Stülke's lab
  • GP2083 (ktrA-ktrB::aphA3 ktrC::tet), available in Jörg Stülke's lab PubMed
  • BKE14510 (ktrC::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCGTGGTGCTCCTTTATA, downstream forward: _UP4_TAGCAGCCAAATAAGCCGTC
  • BKK14510 (ktrC::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCGTGGTGCTCCTTTATA, downstream forward: _UP4_TAGCAGCCAAATAAGCCGTC

    • Expression vector

  • pGP2994: expression of Strep-KtrC in B. subtilis suitable for SPINE (based on pGP380), available in Jörg Stülke's lab
  • pGP2995: expression of KtrC-Strep in B. subtilis suitable for SPINE (based on pGP382), available in Jörg Stülke's lab

    • Expression vectors

  • pGP2907 (N-terminal His-tag, purification from E. coli, in pWH844), available in Jörg Stülke's lab

    • References

    Reviews

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    Original publications

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    Labs working on this gene/protein

  • Erhard Bremer, University of Marburg, Germany homepage